Production of Somatic Cell Nuclear Transfer derived cloned calves in the United Arab Emirates.

Despite the tremendous advancement in somatic cell nuclear transfer (SCNT) camels, generation of cloned calf using SCNT cows is yet to be reported United Arab Emirates (UAE). The present study intended produce calves local environment UAE. We surgically collected ear tissue from two high-performance Holstein (average production 71 liter/ day) and established fibroblast line perform SCNT. Fifty- seven (18.48%) blastocysts were derived 330 fused oocytes for donor A, 51 (19.14%) generated 289 B. used 102 recipients embryo transfer; among those, 53 49 received embryos reconstructed with A B, respectively. single blastocyst media was transferred deep into horn uterus natural cycling recipient by trans cervical method on Day 7; where estrus considered as 0. Non-return (up 42 days), early pregnancy, live birth rate did not differ significantly cells. Four (26.67%) 1 (7.69%) produced Microsatellite analysis 18 bovine-specific loci confirmed perfect match donors calves. This first case Our results could benefit UAE dairy industry supporting locally high- performance replacement heifers. Keywords: Bovine; Cloning; In vitro maturation; Oocytes;